HIV BLOT 2.2, confirmatory kit/18

General Description:
HIV BLOT 2.2, is a qualitative enzyme immunoassay for the in vitro detection of antibodies to
human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2).

Technical specifications:
Technology: Western Blot Assay
Format: Strip in reagent cell, 18 tests
Sample type: Serum, plasma
Sample volume: 20µl
Time to result: 3 hrs

Manufacturer product reference: 11030-018.

Kit content:
1.Nitrocellulose strips, 18
2.Non-reactive control, 1 vial (80µl)
3.Strong reactive control, 1 vial (80µl)
4.Weak reactive control, 1 vial (80µl)
5.Stock buffer concentrate 10x, 1 bottle (20ml)
6.Wash buffer concentrate 20x, 1 bottle (70ml)
7.Conjugate, 1 vial (160µl)
8.Substrate, 1 bottle (100ml)
9.Blotting powder, 10 packets (1gm each)
10.Incubation trays, 2 (9 wells each)
11.Forceps, 1 pair
12.Instructions for use, 1 copy

Items required, but not supplied:
1.Distilled or deionised water, rocking platform (designed with a rocking speed range of 12 to 16 cycles per minute, and which moves through a 5° to 10° tilt to wash membranes evenly), pipettors and tips of appropriate volume, aspirator with sodium hypochlorite trap, 56°C water bath (optional) and sodium hypochlorite for decontamination.
2.Plain/dry vacuum tube (for serum samples), EDTA vacuum tube (for plasma samples), vacuum tube needle, needle holder and centrifuge.
3.Disposable gloves, anti-septic swabs, absorbent paper, protective glasses, timer, container for biohazard disposables and disinfectant (for equipment and surfaces).

Shelf life:
24 months from the date of manufacturing; do not use beyond its expiry date.

Storage and transportation:
Between 2 - 8°C, protected against humidity and direct sunlight. Do not freeze reagents.

Weight and volume:
Estimated weight: 0.5 Kg
Estimated volume: 0,0025 dm³

General recommendations:
1.Only initiate testing when all components of kit reach the temperature of the environment in which the test will be performed.
2.Follow strictly manufacturer instructions for use of the test procedure (especially adherence to reading time).
3.Do not substitute reagents from other kits/batches.
4.Note that serum or plasma samples require supporting equipment and supplies, therefore a laboratory environment is recommended with adequately trained staff.