DENV Detect NS1 ELISA/96

General Description:
DENV Detect NS1 ELISA, is an assay for the detection of NS1 antigen- early detection of Dengue virus. This test will aid in the early diagnosis of Dengue virus even prior to the presence of IgM or IgG antibodies.

Technical specifications:
Technology: Enzyme Immunoassay
Format: Microtitre plate, 96 tests
Sample type: Serum
Sample volume: 50µl
Sensitivity: >85%
Specificity: >97%
Time to result: ~2 hrs

Manufacturer product reference: InBios, DNS1-1.

Items supplied with:
Coated wells: 1 plate of 96 microwells
Dengue NS1 Negative Control, 1 bottle (300µl)
Dengue NS1 Positive Control, 1 bottle (300µl)
Dengue NS1 Cutt-off Control, 1 bottle (300µl)
Sample Diluent for Dengue NS1, 1 bottle (15mls)
Conjugate for Dengue NS1 (100x), 1 bottle (150µl)
Conjugate diluent for Dengue NS1, 1 bottle (12mls)
Wash Buffer (10x), 1 bottle (120ml)
Liquid TMB Substrate, 1 bottle (12mls)
Stop Solution, 1 bottle (6mls)
Instructions for use

Items required, but not supplied:
ELISA Spectrophotometer capable of absorbance measurement at 450 nm, biological or High Grade Water, vacuum pump, automatic plate washer, 37°C Incubator, 1-10μl single-channel pipettors, 50-200μL Single-and Multi-Channel Pipettors, polypropylene tubes or 96 well dilution plates, parafilm and vortex.
For serum samples: Plain/dry vacuum tube, vacuum tube needle, needle holder and centrifuge.
Disposable gloves, anti-septic swabs, absorbent paper, protective glasses, timer, container for biohazard disposables and disinfectant (for equipment and surfaces).

Shelf life:
12 months from the date of manufacturing; do not use beyond its expiry date.
Storage and transportation:
Between 2-8°C, protected against humidity and direct sunlight. Do not freeze reagents.

Packaging and labeling:
Primary packaging: 1 plate of 96 wells from DENV Detect NS1 ELISA, accessories and instructions for use, must be contained in one kit.

Weight and volume:
Estimated weight: 0.45Kg
Estimated volume: 0.3dm³

General recommendations:
Only initiate testing when all components of kit reach the temperature of the environment in which the test will be performed.
Follow strictly manufacturer instructions for use of the test procedure (especially adherence to reading time).
Do not substitute reagents from other kits/batches.
Note that serum or plasma samples require supporting equipment and supplies, therefore a laboratory environment is recommended with adequately trained staff.