S0003474

Genscreen Ultra HIV Ag/Ab ELISA,kit/480


Genscreen Ultra HIV1/2 antibody and HIV1 p24 antigen Enzyme Linked Immunosorbent Assay, ELISA, kit of 480 tests
Indicative Price 183.67 USD

General Description:
Genscreen ULTRA HIV Ag-Ab, is a qualitative enzyme immunoassay kit for the detection of HIV p24 antigen and antibodies to HIV-1 (groups M and O) and HIV-2. This kit can be used for both HIV Ag and HIV Ab screening.

Technical specifications:
Technology: Enzyme Immunoassay
Format: Microtitre plate, 480 tests
Sample type: Serum or plasma
Sample volume: 75µl
Sensitivity: 100%
Specificity: 99.75%
Time to result: 3 hrs

Manufacturer product reference: BIO-RAD, 72388.

Items supplied with:
1.Coated wells, 5 plates of 96 microwells
2.Concentrated washing solution (20X), 1 vial (235 ml)
3.Negative control, 1 vial (2.5 ml)
4.HIV Ab positive control, 1 vial (1 ml)
5.HIV Ag positive control, 1 vial (1 ml)
6.Conjugate 1, 2 vials (2 x 10 ml)
7.Conjugate 2, 2 vials (2 x 30 ml)
8.Conjugate 2 Diluent, 2 vials (2 x 30 ml)
9.Peroxidase substrate buffer, 2 vials (2 x 60 ml)
10.Chromogen, 2 vials (2 x 5 ml)
11.Stopping solution, 3 vials (3 x 28 ml)
12.Instructions for use

Items required, but not supplied:
Distilled or deionised water, sodium hypochlorite (household bleach) and sodium bicarbonate, automatic or semiautomatic, adjustable or preset pipettes or multipipettes to measure and dispense 25μl, 75μl, 80μl and 100μl, graduated cylinders of 25 ml; 100 ml; 1 000 ml capacity, water-bath or equivalent microplate incubator, thermostatically set at 37°C ± 1°C, manual, semiautomatic or automatic microplate washer and microplate reader equipped with 450nm, 490nm and 620-700nm filters.
Plain/dry vacuum tube (for serum samples), EDTA vacuum tube (for plasma samples), vacuum tube needle, needle holder and centrifuge
Disposable gloves, anti-septic swabs, absorbent paper, protective glasses, timer, container for biohazard disposables and disinfectant (for equipment and surfaces).

Shelf life:
18 months from the date of manufacturing; do not use beyond its expiry date.

Weight and volume:
Estimated weight: 1.3 Kg
Estimated volume: 8.107 dm³

Storage and transportation:
Between 2-8°C, protected against humidity and direct sunlight. Do not freeze reagents.

General recommendations:
Only initiate testing when all components of kit reach the temperature of the environment in which the test will be performed.
Follow strictly manufacturer instructions for use of the test procedure (especially adherence to reading time).
Do not substitute reagents from other kits/batches.
Note that serum or plasma samples require supporting equipment and supplies, therefore a laboratory environment is recommended with adequately trained staff.
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