DENV Detect IgM Capture ELISA, kit/96

General Description:
DENV Detect IgM Capture ELISA, is a test for the qualitative detection of IgM antibodies to DENV recombinant antigens (DENRA) for the presumptive clinical laboratory diagnosis of Dengue virus infection.

Technical specifications:
Technology: Enzyme Immunoassay
Format: Microtitre plate, 96 tests
Sample type: Serum
Sample volume: Minimum 4µl
Sensitivity: >90%
Specificity: >90%
Time to result: ~ 3 hrs 50 mins

Manufacturer product reference: InBios, DDMS-1.

Items supplied with:
1.Sample dilution buffer, 1 bottle (25ml)
2.Dengue IgM Negative control, 1vial (50μl)
3.Dengue IgM Positive control, 1 vial (50μl)
4.Dengue recombinant antigen for IgM, 1 bottle (5ml)
5.Normal cell antigen (NCA) for Dengue IgM, 1 bottle (5ml)
6.Enzyme conjugate- HRP for Dengue IgM, 1 bottle (9ml)
7.Wash buffer (10X), 1 bottle (120ml)
8.Enwash, 1 bottle (20ml)
9.Liquid TMB Substrate, 1 bottle (12ml)
10.Stop solution, 1 bottle (9ml)
11.Instructions for use

Items required, but not supplied:
1.ELISA Spectrophotometer capable of absorbance measurement at 450 nm, biological or high-grade water, vacuum pump, plate washer, 37°C incubator without CO2 supply, 1-10 μL single-channel pipettors, 50-200 μL single-and multi-channel pipettors, polypropylene tubes, parafilm and vrtex.
2.For serum samples: Plain/dry vacuum tube, vacuum tube needle, needle holder and centrifuge.
3.Disposable gloves, anti-septic swabs, absorbent paper, protective glasses, timer, container for biohazard disposables and disinfectant (for equipment and surfaces).

Shelf life:
12 months from the date of manufacturing; do not use beyond its expiry date.

Storage and transportation:
Between 2-8°C, protected against humidity and direct sunlight. Do not freeze reagents.

Packaging and labeling:
Primary packaging: 1 plate of 96 wells from DENV Detect IgM Capture ELISA, accessories and instructions for use, must be contained in one kit.

Weight and volume:
Estimated weight: 0.45Kg
Estimated volume: 0.3dm³

General recommendations:
1.Only initiate testing when all components of kit reach the temperature of the environment in which the test will be performed.
2.Follow strictly manufacturer instructions for use of the test procedure (especially adherence to reading time).
3.Do not substitute reagents from other kits/batches.
4.Note that serum or plasma samples require supporting equipment and supplies, therefore a laboratory environment is recommended with adequately trained staff.