S0003885

NovelCoronavirusMultiplexRT-PCRKit,25RXN


Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit (Detection for 3 Genes), 25RXN
Indicative Price : 300.00 USD

General description:
Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the in vitro qualitative detection of 2019 novel coronavirus. RNA in upper respiratory tract specimens and lower respiratory tract specimens and deep cough sputum by real time PCR systems.

Technical specifications:
Principle: RT-PCR--RNA is first transcribed into the complementary DNA (cDNA) by reverse transcriptase from total RNA. The cDNA is then used as a template for the real time PCR.
Test format: RT- PCR Kit
Kit size: 25 reactions
Specimen type: sputum, pharynx swab, BALF
Time to result : 2 hours
Clinical sensitivity: 90.2%
Clinical specificity: 98.28%

Manufacturer product reference:
Shanghai ZJ Bio-Tech Co., Ltd.,RR-0485-02

Items supplied with:
1 vial, Novel CoV (2019-nCoV) Super Mix (513μL)
1 vial, RT-PCR Enzyme Mix (27μL)
1 vial, Novel CoV (2019-nCoV) Internal Control (30μL)
1 vial, Novel CoV (2019-nCoV)Negative Control (400μL)
1 vial, Novel CoV (2019-nCoV)Positive Control (30μL)
1XIFU

Items required, but not supplied:
Instruments:
·7500 Real-time PCR Systems with SDS 2.3 software (Applied Biosystems; catalog #4351104 or #4351105).
·7500 Fast Real-time PCR Systems with SDS 2.3 software (Applied Biosystems; catalog #4351106 or #4351107).
Extraction Kit:
·QIAamp Viral RNA Mini extraction Kit, 52904/52906 (QIAGEN)
Other important Items:
·2 x 96-well -20°C cold-blocks.
·1.5 mL microcentrifuge tubes (Axygen; catalog #MCT-150-C) or equivalent.
·8-strip tubes (Axygen; catalog #PCR-0208-C) or equivalent.
·8-strip caps (Axygen; catalog #PCR-2CP-RT-C) or equivalent.
·Molecular grade water, nuclease-free.
·10% bleach (1:10 dilution of commercial 5.25-6.0% hypochlorite bleach).
·DNAZapTM (Ambion, cat. #AM9890) or equivalent.
·RNAse AwayTM (Fisher Scientific; cat. #21-236-21) or equivalent.
General Lab items:
·Vortexmixer.
·Microcentrifuge.
·Desk top centrifuge with a rotor for 2ml reaction tubes.
·Multichannel micropipettes (disposable).
·Racks for 1.5 mL microcentrifuge tubes.
·Disposable gloves, powderless.
·micropipettes (Disposable).
·Aerosol barrier pipette tips.
·Biohazard waste container.

Shelf life:
6 months from manufacturing date.

Storage & transportation condition:
All reagents should be stored at -20±5°C. All reagents can be used till the expiration date indicated on the kit label. Repeated thawing and freezing (> 3x) should be avoided as this may reduce the sensitivity of the assay. Cool all reagents during the working steps. Super Mix should be stored away from light.

Packaging & labelling:
Primary packaging: All contents of Novel Coronavirus Multiplex RT-PCR Kit of 25RXN
Secondary packaging: One Kit Novel Coronavirus Multiplex RT-PCR Kit, 25RXN

Weight & volume:
Estimated weight: 0.03 Kg
Estimated volume: 0.1485 dm³
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